l6 rat skeletal muscle myoblast cell line  (ATCC)

Journal: Molecules

Article Title: Antiprotozoal Aminosteroids from Pachysandra terminalis

doi: 10.3390/molecules30051093

Figure Lengend Snippet: IC 50 -values (in µg/mL) of the in vitro antiprotozoal ( Plasmodium falciparum , Pf , Trypanosoma brucei rhodesiense , Tbr ) as well as cytotoxic activity (L6 rat skeletal myoblasts) of crude extracts from aerial parts of Pachysandra terminalis and the fractions of the acid/base extraction. Data represent arithmetic means of two independent determinations ± their deviation from the mean. SI: Selectivity indices: IC 50 (Cytotox)/IC 50 ( Pf or Tbr ).

Article Snippet: Cytotoxicity against L-6 rat skeletal myoblasts: The L6 rat skeletal myoblast cell line used for cytotoxicity tests was obtained from the American Type Culture Collection (ATCC, Manassas, VA, USA) as ATCC-CRL-1458 [ ].

Techniques: In Vitro, Activity Assay, Extraction, Residue

Journal: International journal of molecular sciences

Article Title: Identification of Potential Mechanisms of Rk1 Combination with Rg5 in the Treatment of Type II Diabetes Mellitus by Integrating Network Pharmacology and Experimental Validation.

doi: 10.3390/ijms241914828

Figure Lengend Snippet: Figure 5. Effects of Rk1+Rg5 on skeletal muscle glucose uptake in PA-induced L6 cells. (A) Detection of α-sarcomeric actin protein expression in L6 cells by immunofluorescence staining. (B) Cell viability for PA in L6 cells. (C) Screening for optimal induction conditions of PA by glucose consumption. (D) Cell activity for Rk1+Rg5 in PA-induced L6 cells. (E) Glucose consumption for Rk1+Rg5 in IR-L6 cells. (F) Glycogen content for Rk1+Rg5 in IR-L6 cells. (G) Expression of p-Akt in PA-induced L6 cells was analyzed by Western blotting and quantitative results are shown. Data are shown as mean ± SEM (n = 3). *** p < 0.001, ** p < 0.01, * p < 0.05 compared with the con group. ## p < 0.01, # p < 0.05 compared with the PA group. ns: not significant (p > 0.05).

Article Snippet: The rat skeletal muscle myoblast cell line (L6) was purchased from the American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Expressing, Staining, Activity Assay, Western Blot

Journal: Molecules

Article Title: Anti-Cancer and Anti-Inflammatory Activities of a Short Molecule, PS14 Derived from the Virulent Cellulose Binding Domain of Aphanomyces invadans , on Human Laryngeal Epithelial Cells and an In Vivo Zebrafish Embryo Model

doi: 10.3390/molecules27217333

Figure Lengend Snippet: In vitro toxicity of PS14 tested on rat skeletal myoblast cells (L6 cell line) assessed by MTT assay. The bar shows the toxicity values of control (untreated), PC (positive control) Triton X-100 (0.01%) and PS14-treated experimental sample at five different concentrations. The asterix (*) denotes p < 0.05 level of significance compared to the control. Data were presented as mean ± standard deviation (SD) of three independent experiments.

Article Snippet: Rat skeletal myoblast cells (L6 cell line) and human laryngeal cancer cells (Hep-2) were obtained from the Department of Virology, The King Institute of Preventive Medicine and Research, Guindy, Chennai, India.

Techniques: In Vitro, MTT Assay, Control, Positive Control, Standard Deviation